Presence of immunoglobulins, C3 and cytolytic C5b-9 complement components on the surface of erythrocytes from patients with β-thalassaemia/HbE disease

ชื่อเรื่อง

Presence of immunoglobulins, C3 and cytolytic C5b-9 complement components on the surface of erythrocytes from patients with β-thalassaemia/HbE disease See all items with this value

ผู้สร้าง/เจ้าของผลงาน

Malasit, Prida
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Mahasorn, Weerawuth
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Mongkolsapaya, Juthathip
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Singhathong, Benjawan
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Fucharoen, Suthat
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Wasi, Prawase
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Bhakdi, Sucharit See all items with this value

หัวเรื่อง

Hematology See all items with this value

คำอธิบายรายละเอียดของเอกสาร

This study was supported by the USPHS Research Grant HL34408 from the Heart, Lung, and Blood Institute, U.S.A., the Deutsche Forschungsgemeinschaft (SFB 311, project D10) and the Verband der Chemischen Industrie.

บทคัดย่อ

The occurrence of IgG, IgM, IgA, C3 and C5b-9 complement complexes on erythrocytes from 43 patients with β-thalassaemia HbE disease was investigated. Indirect immunoradiometric assays using radioiodinated protein A were employed to quantify the individual components. We confirmed that circulating erythrocytes from thalassaemic patients contained elevated amounts of IgG, and small but significant amounts of C3. In addition, small but significant amounts of C5b-9 were detected. Levels of cell-bound IgG, C3 and C5b-9 were higher in splenectomized versus non-splenectomized patients. The presence of C5b-9 on circulating cells from five splenectomized patients was confirmed by an ELISA employing a monoclonal antibody specific for a C5b-9 neoantigen. When C5b-9 positive cells from two patients were solubilized with detergent and subjected to sucrose density gradient centrifugation, the terminal complexes sedimented as 25–40S macromolecules, thus behaving as membrane C5b-9 complexes. The presence of C8 and C9 in these high molecular weight fractions was directly demonstrated by Western blotting. These results constitute the first demonstration that circulating diseased erythrocytes may carry low numbers of potentially cytolytic C5b-9 complement complexes which may be partly responsible for the known ionic disturbances found in thalassaemic cells. Both bound C3 and C5b-9 could promote removal of diseased cells in the reticuloendothelial system.

ผู้ร่วมงาน

Siriraj Medical Molecular Biology Centre
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Haematology Unit, Department of Medicine, Siriraj Hospital, Mahidol University See all items with this value

Institute of Medical Microbiology, University of Mainz, Mainz, Germany See all items with this value

ปีที่ผลิตเอกสาร

2003 See all items with this value

วันที่บันทึกข้อมูล

2023-05-03

ประเภท

Article See all items with this value

รูปแบบ

pdf See all items with this value

ขนาดหรือจำนวนของเอกสาร

7 pages

ต้นฉบับ/แหล่งที่มาของเอกสาร

British Journal of Haematology ; volume 96, issue 3, page 507-513

ภาษา

en See all items with this value

รหัส หรือตัวบ่งชี้เอกสาร

https://doi.org/10.1046/j.1365-2141.1997.d01-2070.x See all items with this value

ขอบเขต

Health See all items with this value

เจ้าของลิขสิทธิ์

John Wiley & Sons, Inc. See all items with this value

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